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a is definitely the acid dissociation consistent. pH-rate profiles that showed the loss of catalytic action at significant and reduced values of pH daftar naga26 have been suit to equation four, exactly where K

The metallic articles along with the corresponding kinetic parameters with the steel-substituted variants of NagA are detailed in Desk one.

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-trifluoroacetyl substituted substrate is hydrolyzed 26 instances speedier when compared to the purely natural substrate even so the N

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There's no proof to advise that Glu-131 plays any role in catalysis besides to help you coordinate the single divalent cation. The enzyme is level constrained from the cleavage with the amide bond.